fopflash reporter plasmid Search Results


reporter plasmid fopflash  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc reporter plasmid fopflash
Reporter Plasmid Fopflash, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
reporter plasmid fopflash - by Bioz Stars, 2026-03
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ptop/fopflash and renilla reporter plasmids  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc ptop/fopflash and renilla reporter plasmids
Ptop/Fopflash And Renilla Reporter Plasmids, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reporter plasmids containing wild-type (cctttgatc; top flash) or mutated (cctttggcc; fop flash) tcf/lef dna binding sites  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc reporter plasmids containing wild-type (cctttgatc; top flash) or mutated (cctttggcc; fop flash) tcf/lef dna binding sites
Reporter Plasmids Containing Wild Type (Cctttgatc; Top Flash) Or Mutated (Cctttggcc; Fop Flash) Tcf/Lef Dna Binding Sites, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/reporter plasmids containing wild-type (cctttgatc; top flash) or mutated (cctttggcc; fop flash) tcf/lef dna binding sites/product/Upstate Biotechnology Inc
Average 90 stars, based on 1 article reviews
reporter plasmids containing wild-type (cctttgatc; top flash) or mutated (cctttggcc; fop flash) tcf/lef dna binding sites - by Bioz Stars, 2026-03
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top- or fopflash-reporter (firefly luciferase) plasmids  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc top- or fopflash-reporter (firefly luciferase) plasmids
Top Or Fopflash Reporter (Firefly Luciferase) Plasmids, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/top- or fopflash-reporter (firefly luciferase) plasmids/product/Upstate Biotechnology Inc
Average 90 stars, based on 1 article reviews
top- or fopflash-reporter (firefly luciferase) plasmids - by Bioz Stars, 2026-03
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beta-catenin responsive firefly luciferase reporter plasmids fopflash  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc beta-catenin responsive firefly luciferase reporter plasmids fopflash
Increase in <t>beta-catenin</t> expression during E2 treatment does not result in beta-catenin signaling through TCF/LEF response elements in ROS-PG13 cells. Cells were transfected with <t>TopFlash</t> (wild type promoter) and FopFlash (mutant promoter) <t>luciferase</t> reporters in 2% media, and E2 treatment was started three hours later. E2 treatments were staggered during the 48 h interval and all cells were harvested after 48 h after the indicated time of exposure to the hormone. Mutant FopFlash activity was unchanged during the treatment and is not shown. LiCl treatment was carried out to demonstrate the validity of the assay (Inset). Cells were exposed to LiCl or NaCl 24 h after transfection for 16 h. In both these experiments luciferase activity was measured in cell lysates using equal amounts of protein. Experiments represent average ± SEM of triplicate measurements.
Beta Catenin Responsive Firefly Luciferase Reporter Plasmids Fopflash, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/beta-catenin responsive firefly luciferase reporter plasmids fopflash/product/Upstate Biotechnology Inc
Average 90 stars, based on 1 article reviews
beta-catenin responsive firefly luciferase reporter plasmids fopflash - by Bioz Stars, 2026-03
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reporter plasmids 33 mutated tcf-binding site (fopflash)  (Promega)
90
Promega reporter plasmids 33 mutated tcf-binding site (fopflash)
Increase in <t>beta-catenin</t> expression during E2 treatment does not result in beta-catenin signaling through TCF/LEF response elements in ROS-PG13 cells. Cells were transfected with <t>TopFlash</t> (wild type promoter) and FopFlash (mutant promoter) <t>luciferase</t> reporters in 2% media, and E2 treatment was started three hours later. E2 treatments were staggered during the 48 h interval and all cells were harvested after 48 h after the indicated time of exposure to the hormone. Mutant FopFlash activity was unchanged during the treatment and is not shown. LiCl treatment was carried out to demonstrate the validity of the assay (Inset). Cells were exposed to LiCl or NaCl 24 h after transfection for 16 h. In both these experiments luciferase activity was measured in cell lysates using equal amounts of protein. Experiments represent average ± SEM of triplicate measurements.
Reporter Plasmids 33 Mutated Tcf Binding Site (Fopflash), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/reporter plasmids 33 mutated tcf-binding site (fopflash)/product/Promega
Average 90 stars, based on 1 article reviews
reporter plasmids 33 mutated tcf-binding site (fopflash) - by Bioz Stars, 2026-03
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tcf reporter plasmid fopflash  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc tcf reporter plasmid fopflash
Increase in <t>beta-catenin</t> expression during E2 treatment does not result in beta-catenin signaling through TCF/LEF response elements in ROS-PG13 cells. Cells were transfected with <t>TopFlash</t> (wild type promoter) and FopFlash (mutant promoter) <t>luciferase</t> reporters in 2% media, and E2 treatment was started three hours later. E2 treatments were staggered during the 48 h interval and all cells were harvested after 48 h after the indicated time of exposure to the hormone. Mutant FopFlash activity was unchanged during the treatment and is not shown. LiCl treatment was carried out to demonstrate the validity of the assay (Inset). Cells were exposed to LiCl or NaCl 24 h after transfection for 16 h. In both these experiments luciferase activity was measured in cell lysates using equal amounts of protein. Experiments represent average ± SEM of triplicate measurements.
Tcf Reporter Plasmid Fopflash, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tcf reporter plasmid fopflash/product/Upstate Biotechnology Inc
Average 90 stars, based on 1 article reviews
tcf reporter plasmid fopflash - by Bioz Stars, 2026-03
90/100 stars
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fopflash luciferase reporter plasmid with mutated tcf/lef binding sites  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc fopflash luciferase reporter plasmid with mutated tcf/lef binding sites
Increase in <t>beta-catenin</t> expression during E2 treatment does not result in beta-catenin signaling through TCF/LEF response elements in ROS-PG13 cells. Cells were transfected with <t>TopFlash</t> (wild type promoter) and FopFlash (mutant promoter) <t>luciferase</t> reporters in 2% media, and E2 treatment was started three hours later. E2 treatments were staggered during the 48 h interval and all cells were harvested after 48 h after the indicated time of exposure to the hormone. Mutant FopFlash activity was unchanged during the treatment and is not shown. LiCl treatment was carried out to demonstrate the validity of the assay (Inset). Cells were exposed to LiCl or NaCl 24 h after transfection for 16 h. In both these experiments luciferase activity was measured in cell lysates using equal amounts of protein. Experiments represent average ± SEM of triplicate measurements.
Fopflash Luciferase Reporter Plasmid With Mutated Tcf/Lef Binding Sites, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fopflash luciferase reporter plasmid with mutated tcf/lef binding sites/product/Upstate Biotechnology Inc
Average 90 stars, based on 1 article reviews
fopflash luciferase reporter plasmid with mutated tcf/lef binding sites - by Bioz Stars, 2026-03
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fopflash reporter plasmids  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare fopflash reporter plasmids
Increase in <t>beta-catenin</t> expression during E2 treatment does not result in beta-catenin signaling through TCF/LEF response elements in ROS-PG13 cells. Cells were transfected with <t>TopFlash</t> (wild type promoter) and FopFlash (mutant promoter) <t>luciferase</t> reporters in 2% media, and E2 treatment was started three hours later. E2 treatments were staggered during the 48 h interval and all cells were harvested after 48 h after the indicated time of exposure to the hormone. Mutant FopFlash activity was unchanged during the treatment and is not shown. LiCl treatment was carried out to demonstrate the validity of the assay (Inset). Cells were exposed to LiCl or NaCl 24 h after transfection for 16 h. In both these experiments luciferase activity was measured in cell lysates using equal amounts of protein. Experiments represent average ± SEM of triplicate measurements.
Fopflash Reporter Plasmids, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fopflash reporter plasmids/product/Johns Hopkins HealthCare
Average 90 stars, based on 1 article reviews
fopflash reporter plasmids - by Bioz Stars, 2026-03
90/100 stars
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pmlz-fopflash reporter plasmids  (Aventis)
90
Aventis pmlz-fopflash reporter plasmids
Increase in <t>beta-catenin</t> expression during E2 treatment does not result in beta-catenin signaling through TCF/LEF response elements in ROS-PG13 cells. Cells were transfected with <t>TopFlash</t> (wild type promoter) and FopFlash (mutant promoter) <t>luciferase</t> reporters in 2% media, and E2 treatment was started three hours later. E2 treatments were staggered during the 48 h interval and all cells were harvested after 48 h after the indicated time of exposure to the hormone. Mutant FopFlash activity was unchanged during the treatment and is not shown. LiCl treatment was carried out to demonstrate the validity of the assay (Inset). Cells were exposed to LiCl or NaCl 24 h after transfection for 16 h. In both these experiments luciferase activity was measured in cell lysates using equal amounts of protein. Experiments represent average ± SEM of triplicate measurements.
Pmlz Fopflash Reporter Plasmids, supplied by Aventis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmlz-fopflash reporter plasmids/product/Aventis
Average 90 stars, based on 1 article reviews
pmlz-fopflash reporter plasmids - by Bioz Stars, 2026-03
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reporter plasmids fopflash  (Merck KGaA)
90
Merck KGaA reporter plasmids fopflash
Increase in <t>beta-catenin</t> expression during E2 treatment does not result in beta-catenin signaling through TCF/LEF response elements in ROS-PG13 cells. Cells were transfected with <t>TopFlash</t> (wild type promoter) and FopFlash (mutant promoter) <t>luciferase</t> reporters in 2% media, and E2 treatment was started three hours later. E2 treatments were staggered during the 48 h interval and all cells were harvested after 48 h after the indicated time of exposure to the hormone. Mutant FopFlash activity was unchanged during the treatment and is not shown. LiCl treatment was carried out to demonstrate the validity of the assay (Inset). Cells were exposed to LiCl or NaCl 24 h after transfection for 16 h. In both these experiments luciferase activity was measured in cell lysates using equal amounts of protein. Experiments represent average ± SEM of triplicate measurements.
Reporter Plasmids Fopflash, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reporter plasmids fopflash - by Bioz Stars, 2026-03
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Increase in beta-catenin expression during E2 treatment does not result in beta-catenin signaling through TCF/LEF response elements in ROS-PG13 cells. Cells were transfected with TopFlash (wild type promoter) and FopFlash (mutant promoter) luciferase reporters in 2% media, and E2 treatment was started three hours later. E2 treatments were staggered during the 48 h interval and all cells were harvested after 48 h after the indicated time of exposure to the hormone. Mutant FopFlash activity was unchanged during the treatment and is not shown. LiCl treatment was carried out to demonstrate the validity of the assay (Inset). Cells were exposed to LiCl or NaCl 24 h after transfection for 16 h. In both these experiments luciferase activity was measured in cell lysates using equal amounts of protein. Experiments represent average ± SEM of triplicate measurements.

Journal: Cancer Cell International

Article Title: P53 and Beta-Catenin Activity during Estrogen treatment of Osteoblasts

doi: 10.1186/1475-2867-5-24

Figure Lengend Snippet: Increase in beta-catenin expression during E2 treatment does not result in beta-catenin signaling through TCF/LEF response elements in ROS-PG13 cells. Cells were transfected with TopFlash (wild type promoter) and FopFlash (mutant promoter) luciferase reporters in 2% media, and E2 treatment was started three hours later. E2 treatments were staggered during the 48 h interval and all cells were harvested after 48 h after the indicated time of exposure to the hormone. Mutant FopFlash activity was unchanged during the treatment and is not shown. LiCl treatment was carried out to demonstrate the validity of the assay (Inset). Cells were exposed to LiCl or NaCl 24 h after transfection for 16 h. In both these experiments luciferase activity was measured in cell lysates using equal amounts of protein. Experiments represent average ± SEM of triplicate measurements.

Article Snippet: For reporter assays, cells were transfected with the beta-catenin responsive firefly luciferase reporter plasmids TopFlash (wild type promoter) or FopFlash (Mutant promoter) (Upstate Biotechnologies) for 48 h. Three hours after transfection, cells received 17-beta estradiol to a concentration of 10–11 M for the times indicated.

Techniques: Expressing, Transfection, Mutagenesis, Luciferase, Activity Assay